Visualization of the Nucleolus Using 5′ Ethynyl Uridine

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00139586" target="_blank" >RIV/00216224:14740/23:00139586 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007/978-1-0716-3226-0_24" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-3226-0_24</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-3226-0_24" target="_blank" >10.1007/978-1-0716-3226-0_24</a>

Result language
angličtina
Original language name
Visualization of the Nucleolus Using 5′ Ethynyl Uridine
Original language description
Labeling of the nucleolus in Arabidopsis thaliana can be achieved by incorporation of 5′-ethynyl uridine (EU) into bulk RNA. Although EU does not selectively label the nucleolus, the abundance of ribosomal transcripts results in the predominant accumulation of the signal in the nucleolus. Ethynyl uridine has the advantage of being detected via Click-iT chemistry providing a specific signal and low background. While the protocol presented here employs fluorescent dye and allows visualization of the nucleolus by microscopy, this method can also be used for other downstream applications. Though we tested nucleolar labeling only in A. thaliana, in principle it can be applied to other plant species.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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