Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00101169" target="_blank" >RIV/00216224:14740/18:00101169 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.snb.2018.08.055" target="_blank" >http://dx.doi.org/10.1016/j.snb.2018.08.055</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2018.08.055" target="_blank" >10.1016/j.snb.2018.08.055</a>

Result language

angličtina

Original language name

Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella

Original language description

We report on the first successful application of nitrogen-containing functional surfaces prepared by plasma polymerization in surface plasmon resonance (SPR) immunosensing. The plasma polymers (PPs) were deposited from cyclopropylamine (CPA) vapors onto gold surfaces of SPR and quartz crystal microbalance (QCM) sensors. It provided an excellent platform for a stable immobilization of antibodies using glutaraldehyde (GA) activation. The performance of the SPR immunosensor was proven by a model pair of monoclonal antibody (Ab) AL-01 and human serum albumin as well as by the selective detection of bacterial pathogen Salmonella Typhimurium using the immobilized anti-Salmonella Ab. A baseline drift of the measured SPR signal, caused by the long-term reactivity of CPA plasma polymers, was well stabilized by 18 h immersion in phosphate buffered saline prior to the GA activation. The immunosensors can be successfully regenerated several times using 10 mM NaOH. The limit of detection of 10^5 CFU/mL and a wide linear response were achieved for Salmonella. The assay parameters are comparable to the conventional label-free methods. However, the replacement of conventional immobilization matrix with the CPA PPs offers an advantage of excellent adhesion to gold QCM and SPR surfaces achieved by fast and eco-friendly procedure compatible with the vacuum deposition of gold.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors and Actuators B: Chemical

ISSN

0925-4005

e-ISSN

—

Volume of the periodical

276

Issue of the periodical within the volume

DEC

Country of publishing house

CH - SWITZERLAND

Number of pages

9

Pages from-to

447-455

UT code for WoS article

000444637600056

EID of the result in the Scopus database

2-s2.0-85052620714