NMR spectroscopy of nucleic acids in human cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00101760" target="_blank" >RIV/00216224:14740/18:00101760 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

NMR spectroscopy of nucleic acids in human cells

Original language description

Nucleic acids are abundant biopolymers responsible for encoding, transmitting, and expressing genetic information. A milestone towards detail mechanistic understanding of biological functionality of nucleic acids is the characterization of their physiological structure and dynamics. There is a number of well­established biophysical techniques allowing characterization of nucleic acids in terma of their structure and/or dynamics including atomic­resolution methods such as X­ray diffraction (XRD) and solution nuclear magnetic resonance (NMR) spectroscopy. However, the structural information delivered by these methods often strongly depends on (subjectively chosen) experimental/environmental conditions and used instrumental setup. The consideration of the inherent sensitivity of nucleic acids structure to environmental factors has indicated that delineating of physiologically relevant quantitative structure­activity relationships for nucleic acids might only be possible with the use structural data derived under environmental conditions that are as close as possible to native conditions in vivo. Recently, number of methods has been developed for characterization of nucleic acid structure under physiological conditions in living cells. Here, I will illustrate potential of in­cell NMR spectroscopy for characterization of nucleic acid structure, stability, and interactions with drug like molecules in the native environment of the nuclei of living human cells

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA16-10504S" target="_blank" >GA16-10504S: Characterization of nucleic acids structure in complex environment of living cells using high-resolution NMR spectroscopy</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů