In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00535922" target="_blank" >RIV/68081707:_____/19:00535922 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/19:00108250
Result on the web
<a href="https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-9666-7_25" target="_blank" >10.1007/978-1-4939-9666-7_25</a>
Alternative languages
Result language
angličtina
Original language name
In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes
Original language description
G-quadruplexes are inherently polymorphic nucleic acid structures. Their folding topology depends on the nucleic acid primary sequence and on physical-chemical environmental factors. Hence, it remains unclear if a G-quadruplex topology determined in the test tube (in vitro) will also form in vivo. Characterization of G-quadruplexes in their native environment has been proposed as an efficient strategy to tackle this issue. So far, characterization of G-quadruplex structures in living cells has relied exclusively on the use of Xenopus laevis oocytes as a eukaryotic cell model system. Here, we describe the protocol for the preparation of X. laevis oocytes for studies of G-quadruplexes as well as other nucleic acids motifs under native conditions using in-cell NMR spectroscopy.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods in Molecular Biology
ISSN
1064-3745
e-ISSN
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Volume of the periodical
2035
Issue of the periodical within the volume
2019
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
397-405
UT code for WoS article
000520558900026
EID of the result in the Scopus database
2-s2.0-85071465198