Functional Characterization of SMG7 Paralogs in Arabidopsis thaliana

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00101791" target="_blank" >RIV/00216224:14740/18:00101791 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3389/fpls.2018.01602" target="_blank" >http://dx.doi.org/10.3389/fpls.2018.01602</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2018.01602" target="_blank" >10.3389/fpls.2018.01602</a>

Result language

angličtina

Original language name

Functional Characterization of SMG7 Paralogs in Arabidopsis thaliana

Original language description

SMG7 proteins are evolutionary conserved across eukaryotes and primarily known for their function in nonsense mediated RNA decay (NMD). In contrast to other NMD factors, SMG7 proteins underwent independent expansions during evolution indicating their propensity to adopt novel functions. Here we characterized SMG7 and SMG7-like (SMG7L) paralogs in Arabidopsis thaliana. SMG7 retained its role in NMD and additionally appears to have acquired another function in meiosis. We inactivated SMG7 by CRISPR/Cas9 mutagenesis and showed that, in contrast to our previous report, SMG7 is not an essential gene in Arabidopsis. Furthermore, our data indicate that the N-terminal phosphoserine-binding domain is required for both NMD and meiosis. Phenotypic analysis of SMG7 and SMG7L double mutants did not indicate any functional redundancy between the two genes, suggesting neofunctionalization of SMG7L. Finally, protein sequence comparison together with a phenotyping of T-DNA insertion mutants identified several conserved regions specific for SMG7 that may underlie its role in NMD and meiosis. This information provides a framework for deciphering the non-canonical functions of SMG7-family proteins.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

NOV

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

1602

UT code for WoS article

000449282400001

EID of the result in the Scopus database

2-s2.0-85058818249