Multivalency regulates activity in an intrinsically disordered transcription factor
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00106665" target="_blank" >RIV/00216224:14740/18:00106665 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.7554/eLife.36258" target="_blank" >http://dx.doi.org/10.7554/eLife.36258</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7554/eLife.36258" target="_blank" >10.7554/eLife.36258</a>
Alternative languages
Result language
angličtina
Original language name
Multivalency regulates activity in an intrinsically disordered transcription factor
Original language description
The transcription factor ASCIZ (ATMIN, ZNF822) has an unusually high number of recognition motifs for the product of its main target gene, the hub protein LC8 (DYNLL1). Using a combination of biophysical methods, structural analysis by NMR and electron microscopy, and cellular transcription assays, we developed a model that proposes a concerted role of intrinsic disorder and multiple LC8 binding events in regulating LC8 transcription. We demonstrate that the long intrinsically disordered C -terminal domain of ASCIZ binds LC8 to form a dynamic ensemble of complexes with a gradient of transcriptional activity that is inversely proportional to LC8 occupancy. The preference for low occupancy complexes at saturating LC8 concentrations with both human and Drosophila ASCIZ indicates that negative cooperativity is an important feature of ASCIZ-LC8 interactions. The prevalence of intrinsic disorder and multivalency among transcription factors suggests that formation of heterogeneous, dynamic complexes is a widespread mechanism for tuning transcriptional regulation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
elife
ISSN
2050-084X
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
MAY
Country of publishing house
GB - UNITED KINGDOM
Number of pages
28
Pages from-to
1-28
UT code for WoS article
000432725100001
EID of the result in the Scopus database
2-s2.0-85051995414