Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00107272" target="_blank" >RIV/00216224:14740/19:00107272 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/19:00071044
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0956566318308315?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0956566318308315?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bios.2018.10.021" target="_blank" >10.1016/j.bios.2018.10.021</a>
Alternative languages
Result language
angličtina
Original language name
Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models
Original language description
Cardiomyocytes (CM) placed on microelectrode array (MEA) were simultaneously probed with cantilever from atomic force microscope (AFM) system. This electric,nanomechanical combination in real time recorded beating force of the CMs cluster and the triggering electric events. Such "organ-on-a-chip" represents a tool for drug development and disease modeling. The human pluripotent stem cells included the WT embryonic line CCTL14 and the induced dystrophin deficient line reprogrammed from fibroblasts of a patient affected by Duchenne Muscular Dystrophy (DMD, complete loss of dystrophin expression). Both were differentiated to CMs and employed with the AFM/MEA platform for diseased CMs’ drug response testing and DMD characterization. The dependence of cardiac parameters on extracellular Ca2+ was studied. The differential evaluation explained the observed effects despite variability of biological samples. The beta-adrenergic stimulation (isoproterenol) and antagonist trials (verapamil) addressed ionotropic and chronotropic cell line-dependent features. For the first time, a distinctive beating-force relation for DMD CMs was measured on the 3D cardiac in vitro model.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biosensors & bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices
ISSN
0956-5663
e-ISSN
1873-4235
Volume of the periodical
124
Issue of the periodical within the volume
JAN 15 2019
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
129-135
UT code for WoS article
000451935500016
EID of the result in the Scopus database
2-s2.0-85055205500