Structure and genome ejection mechanism of Staphylococcus aureus phage P68
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00107744" target="_blank" >RIV/00216224:14740/19:00107744 - isvavai.cz</a>
Result on the web
<a href="https://advances.sciencemag.org/content/5/10/eaaw7414" target="_blank" >https://advances.sciencemag.org/content/5/10/eaaw7414</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1126/sciadv.aaw7414" target="_blank" >10.1126/sciadv.aaw7414</a>
Alternative languages
Result language
angličtina
Original language name
Structure and genome ejection mechanism of Staphylococcus aureus phage P68
Original language description
Phages infecting Staphylococcus aureus can be used as therapeutics against antibiotic-resistant bacterial infections. However, there is limited information about the mechanism of genome delivery of phages that infect Gram-positive bacteria. Here, we present the structures of native S. aureus phage P68, genome ejection intermediate, and empty particle. The P68 head contains 72 subunits of inner core protein, 15 of which bind to and alter the structure of adjacent major capsid proteins and thus specify attachment sites for head fibers. Unlike in the previously studied phages, the head fibers of P68 enable its virion to position itself at the cell surface for genome delivery. The unique interaction of one end of P68 DNA with one of the 12 portal protein subunits is disrupted before the genome ejection. The inner core proteins are released together with the DNA and enable the translocation of phage genome across the bacterial membrane into the cytoplasm.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Science advances
ISSN
2375-2548
e-ISSN
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Volume of the periodical
5
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
„eaaw7414“
UT code for WoS article
000491132700068
EID of the result in the Scopus database
2-s2.0-85073872322