Signals trigger state-specific transcriptional programs to support diversity and homeostasis in immune cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00113240" target="_blank" >RIV/00216224:14740/19:00113240 - isvavai.cz</a>
Result on the web
<a href="https://stke.sciencemag.org/content/12/581/eaao5820" target="_blank" >https://stke.sciencemag.org/content/12/581/eaao5820</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1126/scisignal.aao5820" target="_blank" >10.1126/scisignal.aao5820</a>
Alternative languages
Result language
angličtina
Original language name
Signals trigger state-specific transcriptional programs to support diversity and homeostasis in immune cells
Original language description
Macrophages play key roles in the immune systems of humans and other mammals. Here, we performed single-cell analyses of the mRNAs and proteins of human macrophages to compare their responses to the signaling molecules lipopolysaccharide (LPS), a component of Gram-negative bacteria, and palmitate (PAL), a free fatty acid. We found that, although both molecules signal through the cell surface protein Toll-like receptor 4 (TLR4), they stimulated the expression of different genes, resulting in specific pro- and anti-inflammatory cellular states for each signal. The effects of the glucocorticoid receptor, which antagonizes LPS signaling, and cyclic AMP-dependent transcription factor 3, which inhibits PAL-induced inflammation, on inflammatory response seemed largely determined by digital on-off events. Furthermore, the quantification of transcriptional variance and signaling entropy enabled the identification of cell state-specific deregulated molecular pathways. These data suggest that the preservation of signaling in distinct cells might confer diversity on macrophage populations essential to maintaining major cellular functions.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
SCIENCE SIGNALING
ISSN
1945-0877
e-ISSN
—
Volume of the periodical
12
Issue of the periodical within the volume
581
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
1-16
UT code for WoS article
000467963000001
EID of the result in the Scopus database
2-s2.0-85066279813