A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00113325" target="_blank" >RIV/00216224:14740/19:00113325 - isvavai.cz</a>
Result on the web
<a href="http://sro.sussex.ac.uk/id/eprint/84897/1/gkz600.pdf" target="_blank" >http://sro.sussex.ac.uk/id/eprint/84897/1/gkz600.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkz600" target="_blank" >10.1093/nar/gkz600</a>
Alternative languages
Result language
angličtina
Original language name
A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling
Original language description
Translation is controlled by numerous accessory proteins and translation factors. In the yeast Saccharomyces cerevisiae, translation elongation requires an essential elongation factor, the ABCF ATPase eEF3. A closely related protein, New1, is encoded by a non-essential gene with cold sensitivity and ribosome assembly defect knock-out phenotypes. Since the exact molecular function of New1 is unknown, it is unclear if the ribosome assembly defect is direct, i.e. New1 is a bona fide assembly factor, or indirect, for instance due to a defect in protein synthesis. To investigate this, we employed yeast genetics, cryo-electron microscopy (cryo-EM) and ribosome profiling (Ribo-Seq) to interrogate the molecular function of New1. Overexpression of New1 rescues the inviability of a yeast strain lacking the otherwise strictly essential translation factor eEF3. The structure of the ATPase-deficient (EQ(2)) New1 mutant locked on the 80S ribosome reveals that New1 binds analogously to the ribosome as eEF3. Finally, Ribo-Seq analysis revealed that loss of New1 leads to ribosome queuing upstream of 3'-terminal lysine and arginine codons, including those genes encoding proteins of the cytoplasmic translational machinery. Our results suggest that New1 is a translation factor that fine-tunes the efficiency of translation termination or ribosome recycling.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LM2015043" target="_blank" >LM2015043: Czech Infrastructure for Integrative Structural Biology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nucleic acids research
ISSN
0305-1048
e-ISSN
1362-4962
Volume of the periodical
47
Issue of the periodical within the volume
16
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
8807-8820
UT code for WoS article
000490576900040
EID of the result in the Scopus database
2-s2.0-85073311572