Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00124438" target="_blank" >RIV/00216224:14740/21:00124438 - isvavai.cz</a>
Result on the web
<a href="https://www.embopress.org/doi/full/10.15252/embj.2020106449" target="_blank" >https://www.embopress.org/doi/full/10.15252/embj.2020106449</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15252/embj.2020106449" target="_blank" >10.15252/embj.2020106449</a>
Alternative languages
Result language
angličtina
Original language name
Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation
Original language description
In addition to the conserved translation elongation factors eEF1A and eEF2, fungi require a third essential elongation factor, eEF3. While eEF3 has been implicated in tRNA binding and release at the ribosomal A and E sites, its exact mechanism of action is unclear. Here, we show that eEF3 acts at the mRNA-tRNA translocation step by promoting the dissociation of the tRNA from the E site, but independent of aminoacyl-tRNA recruitment to the A site. Depletion of eEF3 in vivo leads to a general slowdown in translation elongation due to accumulation of ribosomes with an occupied A site. Cryo-EM analysis of native eEF3-ribosome complexes shows that eEF3 facilitates late steps of translocation by favoring non-rotated ribosomal states, as well as by opening the L1 stalk to release the E-site tRNA. Additionally, our analysis provides structural insights into novel translation elongation states, enabling presentation of a revised yeast translation elongation cycle.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
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Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
EMBO Journal
ISSN
0261-4189
e-ISSN
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Volume of the periodical
40
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
1-20
UT code for WoS article
000615851800001
EID of the result in the Scopus database
2-s2.0-85100560703