Structural basis for+1 ribosomal frameshifting during EF-G-catalyzed translocation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00119665" target="_blank" >RIV/00216224:14740/21:00119665 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41467-021-24911-1" target="_blank" >https://www.nature.com/articles/s41467-021-24911-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-021-24911-1" target="_blank" >10.1038/s41467-021-24911-1</a>
Alternative languages
Result language
angličtina
Original language name
Structural basis for+1 ribosomal frameshifting during EF-G-catalyzed translocation
Original language description
Frameshifting of mRNA during translation provides a strategy to expand the coding repertoire of cells and viruses. How and where in the elongation cycle +1-frameshifting occurs remains poorly understood. We describe seven similar to 3.5-angstrom-resolution cryo-EM structures of 70S ribosome complexes, allowing visualization of elongation and translocation by the GTPase elongation factor G (EF-G). Four structures with a + 1-frameshifting-prone mRNA reveal that frameshifting takes place during translocation of tRNA and mRNA. Prior to EF-G binding, the pre-translocation complex features an in-frame tRNA-mRNA pairing in the A site. In the partially translocated structure with EF-G center dot GDPCP, the tRNA shifts to the +1-frame near the P site, rendering the freed mRNA base to bulge between the P and E sites and to stack on the 16S rRNA nucleotide G926. The ribosome remains frameshifted in the nearly post-translocation state. Our findings demonstrate that the ribosome and EF-G cooperate to induce +1 frameshifting during tRNA-mRNA translocation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GJ20-16013Y" target="_blank" >GJ20-16013Y: Simultaneous gene transcription and translation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Communications
ISSN
2041-1723
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
12
Pages from-to
4644
UT code for WoS article
000684299000001
EID of the result in the Scopus database
2-s2.0-85111641609