Cryo-EM ensemble captures EF-G mediated ribosomal translocation in action
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00122989" target="_blank" >RIV/00216224:14740/21:00122989 - isvavai.cz</a>
Result on the web
<a href="https://csbmb2021.cz/detailed-programme/" target="_blank" >https://csbmb2021.cz/detailed-programme/</a>
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Cryo-EM ensemble captures EF-G mediated ribosomal translocation in action
Original language description
During protein synthesis, transfer RNAs (tRNAs) and messenger RNA (mRNA) codons are translocated within the ribosome from the A to P to E sites, respectively. Translocation of tRNAs anticodons and mRNA along the small ribosomal 30S subunit is catalyzed by a conserved GTPase, elongation factor G(EF-G) in bacteria. The structural mechanism how the ribosome and EF-G maintain the open reading frame has not been visualized because the rapid GTP hydrolysis step has prevented the capture of authentic EF-G bound structural intermediates. Here, we present our single particle cryo-EM study aimed at characterizing translocation without using EF-G mutations or antibiotics. We report newly described intermediate structural state, which visualized the transition of two tRNAs from the A and P to P and E sites during translocation. The structure visualizes how nearly rigid EF-G rectifies inherent and spontaneous ribosomal dynamics into tRNA and mRNA translocation. This work therefore uncovers a missing link in the understanding of the synchronous movement of tRNAs and mRNA during translation elongation.
Czech name
—
Czech description
—
Classification
Type
O - Miscellaneous
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LL2008" target="_blank" >LL2008: Crosstalk between transcription and translation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů