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Cryo-EM ensemble captures EF-G mediated ribosomal translocation in action

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00122989" target="_blank" >RIV/00216224:14740/21:00122989 - isvavai.cz</a>

  • Result on the web

    <a href="https://csbmb2021.cz/detailed-programme/" target="_blank" >https://csbmb2021.cz/detailed-programme/</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Cryo-EM ensemble captures EF-G mediated ribosomal translocation in action

  • Original language description

    During protein synthesis, transfer RNAs (tRNAs) and messenger RNA (mRNA) codons are translocated within the ribosome from the A to P to E sites, respectively. Translocation of tRNAs anticodons and mRNA along the small ribosomal 30S subunit is catalyzed by a conserved GTPase, elongation factor G(EF-G) in bacteria. The structural mechanism how the ribosome and EF-G maintain the open reading frame has not been visualized because the rapid GTP hydrolysis step has prevented the capture of authentic EF-G bound structural intermediates. Here, we present our single particle cryo-EM study aimed at characterizing translocation without using EF-G mutations or antibiotics. We report newly described intermediate structural state, which visualized the transition of two tRNAs from the A and P to P and E sites during translocation. The structure visualizes how nearly rigid EF-G rectifies inherent and spontaneous ribosomal dynamics into tRNA and mRNA translocation. This work therefore uncovers a missing link in the understanding of the synchronous movement of tRNAs and mRNA during translation elongation.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/LL2008" target="_blank" >LL2008: Crosstalk between transcription and translation</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů