Transcriptomic and Proteomic Insights into Amborella trichopoda Male Gametophyte Functions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00118232" target="_blank" >RIV/00216224:14740/20:00118232 - isvavai.cz</a>
Result on the web
<a href="http://www.plantphysiol.org/content/plantphysiol/184/4/1640.full.pdf" target="_blank" >http://www.plantphysiol.org/content/plantphysiol/184/4/1640.full.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1104/pp.20.00837" target="_blank" >10.1104/pp.20.00837</a>
Alternative languages
Result language
angličtina
Original language name
Transcriptomic and Proteomic Insights into Amborella trichopoda Male Gametophyte Functions
Original language description
Transcriptomic and proteomic data from the basal angiosperm Amborella provide a resource to understand the evolution of male gametophyte functions in flowering plants. Flowering plants (angiosperms) are characterized by pollen tubes (PTs; male gametophytes) carrying two immobile sperm cells that grow over long distances through the carpel toward the ovules, where double fertilization is executed. It is not understood how these reproductive structures evolved, which genes occur de novo in male gametophytes of angiosperms, and to which extent PT functions are conserved among angiosperms. To contribute to a deeper understanding of the evolution of gametophyte functions, we generated RNA sequencing data from seven reproductive and two vegetative control tissues of the basal angiosperm Amborella trichopoda and complemented these with proteomic data of pollen grains (PGs) and PTs. The eudicot model plant Arabidopsis (Arabidopsis thaliana) served as a reference organism for data analysis, as more than 200 genes have been associated with male gametophyte functions in this species. We describe methods to collect bicellular A. trichopoda PGs, to induce their germination in vitro, and to monitor PT growth and germ cell division. Transcriptomic and proteomic analyses indicate that A. trichopoda PGs are prepared for germination requiring lipids, energy, but likely also reactive oxygen species, while PTs are especially characterized by catabolic/biosynthetic and transport processes including cell wall biosynthesis and gene regulation. Notably, a number of pollen-specific genes were lacking in Arabidopsis, and the number of genes involved in pollen signaling is significantly reduced in A. trichopoda. In conclusion, we provide insight into male gametophyte functions of the most basal angiosperm and establish a valuable resource for future studies on the evolution of flowering plants.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Physiology
ISSN
0032-0889
e-ISSN
1532-2548
Volume of the periodical
184
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
1640-1657
UT code for WoS article
000601303200010
EID of the result in the Scopus database
2-s2.0-85096923176