Primary and secondary anti-viral response captured by the dynamics and phenotype of individual T cell clones
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00118340" target="_blank" >RIV/00216224:14740/20:00118340 - isvavai.cz</a>
Result on the web
<a href="https://elifesciences.org/articles/53704" target="_blank" >https://elifesciences.org/articles/53704</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7554/eLife.53704" target="_blank" >10.7554/eLife.53704</a>
Alternative languages
Result language
angličtina
Original language name
Primary and secondary anti-viral response captured by the dynamics and phenotype of individual T cell clones
Original language description
The diverse repertoire of T-cell receptors (TCR) plays a key role in the adaptive immune response to infections. Using TCR alpha and beta repertoire sequencing for T-cell subsets, as well as single-cell RNAseq and TCRseq, we track the concentrations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever immunization - the model for acute infection in humans - showing their large diversity. We confirm the secondary response is an order of magnitude weaker, albeit similar to 10 days faster than the primary one. Estimating the fraction of the T-cell response directed against the single immunodominant epitope, we identify the sequence features of TCRs that define the high precursor frequency of the two major TCR motifs specific for this particular epitope. We also show the consistency of clonal expansion dynamics between bulk alpha and beta repertoires, using a new methodology to reconstruct alpha-beta pairings from clonal trajectories.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
elife
ISSN
2050-084X
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
FEB
Country of publishing house
GB - UNITED KINGDOM
Number of pages
21
Pages from-to
53704
UT code for WoS article
000518830000001
EID of the result in the Scopus database
2-s2.0-85081944059