TCRs with segment TRAV9-2 or a CDR3 histidine are overrepresented among nickel-specific CD4+T cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00118355" target="_blank" >RIV/00216224:14740/20:00118355 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/all.14322" target="_blank" >https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/all.14322</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/all.14322" target="_blank" >10.1111/all.14322</a>
Alternative languages
Result language
angličtina
Original language name
TCRs with segment TRAV9-2 or a CDR3 histidine are overrepresented among nickel-specific CD4+T cells
Original language description
Background Nickel is the most frequent cause of T cell-mediated allergic contact dermatitis worldwide. In vitro, CD4+ T cells from all donors respond to nickel but the involved alpha beta T cell receptor (TCR) repertoire has not been comprehensively analyzed. Methods We introduce CD154 (CD40L) upregulation as a fast, unbiased, and quantitative method to detect nickel-specific CD4+ T cells ex vivo in blood of clinically characterized allergic and non allergic donors. Naive (CCR7+ CD45RA+) and memory (not naive) CD154+ CD4+ T cells were analyzed by flow cytometry after 5 hours of stimulation with 200 mu mol/L NiSO4., TCR alpha- and beta-chains of sorted nickel-specific and control cells were studied by high-throughput sequencing. Results Stimulation of PBMCs with NiSO4 induced CD154 expression on similar to 0.1% (mean) of naive and memory CD4+ T cells. In allergic donors with recent positive patch test, memory frequencies further increased similar to 13-fold and were associated with markers of in vivo activation. CD154 expression was TCR-mediated since single clones could be specifically restimulated. Among nickel-specific CD4+ T cells of allergic and non allergic donors, TCRs expressing the alpha-chain segment TRAV9-2 or a histidine in their alpha- or beta-chain complementarity determining region 3 (CDR3) were highly overrepresented. Conclusions Induced CD154 expression represents a reliable method to study nickel-specific CD4+ T cells. TCRs with particular features respond in all donors, while strongly increased blood frequencies indicate nickel allergy for some donors. Our approach may be extended to other contact allergens for the further development of diagnostic and predictive in vitro tests.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30225 - Allergy
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Allergy
ISSN
0105-4538
e-ISSN
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Volume of the periodical
75
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
2574-2586
UT code for WoS article
000531897200001
EID of the result in the Scopus database
2-s2.0-85084483210