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Cdk11 is a Novel Player in Non-coding RNA Expression

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00118404" target="_blank" >RIV/00216224:14740/20:00118404 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Cdk11 is a Novel Player in Non-coding RNA Expression

  • Original language description

    Cdk11 is a poorly-characterized cyclin-dependent kinase with described functions in transcription of mRNA, alternative splicing and 3’end processing of HIV mRNA. Our aim was to better define its cellular roles via identification of novel interacting partners and candidate substrates combined with data from microarray experiment after knock-down of Cdk11. We utilized BioID method to probe for relevant proteins connected to Cdk11. Microarray data showed differential expression of a subset of non-coding genes. Proteins implicated in control of their expression and processing were identified in BioID screen as candidate interacting partners of Cdk11. We utilized also Cdk11 analog-sensitive mutant (designed using Shokat’s lab approach) to test the candidate interacting partners as substrates of Cdk11 in vitro. Altogether, our data uncover novel role of Cdk11 in the expression of subset of non-coding RNAs

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů