Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F20%3A00121457" target="_blank" >RIV/00216224:14740/20:00121457 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/20:43917906 RIV/00216305:26620/20:PU136753

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0925400520304913?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0925400520304913?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2020.128141" target="_blank" >10.1016/j.snb.2020.128141</a>

Result language

angličtina

Original language name

Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode

Original language description

A simple and reproducible electroanalytical sensor using antimony trioxide modified-carbon paste electrode (Sb2O3-CPE) as sensing element was employed for the first time for simultaneous determination of glutathione (GSH) and glutathione disulphide (GSSG). The electrochemical response of Sb2O3-CPE was characterized by cyclic voltammetry and square-wave voltammetry. Scanning electron microscopy (SEM), scanning electrochemical microscopy (SECM) and Raman mapping were used to characterize the morphology and surface modification. A significant enhancement in the peak current response of GSH and GSSG accompanied with a good separation of the peaks were obtained at the modified electrode compared to the bare CPE. GSH and GSSG exhibited well-defined oxidation peaks at +1.08 V and +1.36 V (vs. Ag/AgCl, 3 mol/L) in Britton-Robinson buffer at pH 6.0. Parameters such as used supporting electrolyte and its pH value, amplitude and frequency were optimized for square-wave voltammetric determination. Under optimal conditions, voltammetric responses were linear in the concentration range of 2-200 mu mol/L for GSH and GSSG. The limits of detection were 0.34 mu mol/L and 0.10 mu mol/L for GSH and GSSG, respectively. Moreover, the proposed sensor also showed good reproducibility, stability and selectivity. Finally, the proposed sensor was applied for the simultaneous determination of GSH and GSSG in urine samples with satisfactory results.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

—

Continuities

—

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors and Actuators B: Chemical

ISSN

0925-4005

e-ISSN

—

Volume of the periodical

318

Issue of the periodical within the volume

SEP

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

128141

UT code for WoS article

000539189500001

EID of the result in the Scopus database

2-s2.0-85084634181