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Super-resolution microscopy of chromatin fibers and quantitative DNA methylation analysis of DNA fiber preparations

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00119668" target="_blank" >RIV/00216224:14740/21:00119668 - isvavai.cz</a>

  • Alternative codes found

    RIV/00159816:_____/21:00075086 RIV/00216208:11310/21:10442523

  • Result on the web

    <a href="https://journals.biologists.com/jcs/article/134/15/jcs258374/271206/Super-resolution-microscopy-of-chromatin-fibers" target="_blank" >https://journals.biologists.com/jcs/article/134/15/jcs258374/271206/Super-resolution-microscopy-of-chromatin-fibers</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1242/jcs.258374" target="_blank" >10.1242/jcs.258374</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Super-resolution microscopy of chromatin fibers and quantitative DNA methylation analysis of DNA fiber preparations

  • Original language description

    Analysis of histone variants and epigenetic marks is dominated by genome-wide approaches in the form of chromatin immunoprecipitation-sequencing (ChIP-seq) and related methods. Although uncontested in their value for single-copy genes, mapping the chromatin of DNA repeats is problematic for biochemical techniques that involve averaging of cell populations or analysis of clusters of tandem repeats in a single-cell analysis. Extending chromatin and DNA fibers allows us to study the epigenetics of individual repeats in their specific chromosomal context, and thus constitutes an important tool for gaining a complete understanding of the epigenetic organization of genomes. We report that using an optimized fiber extension protocol is essential in order to obtain more reproducible data and to minimize the clustering of fibers. We also demonstrate that the use of super-resolution microscopy is important for reliable evaluation of the distribution of histone modifications on individual fibers. Furthermore, we introduce a custom script for the analysis of methylation levels on DNA fibers and apply it to map the methylation of telomeres, ribosomal genes and centromeres.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10601 - Cell biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Cell Science

  • ISSN

    0021-9533

  • e-ISSN

    1477-9137

  • Volume of the periodical

    134

  • Issue of the periodical within the volume

    15

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    14

  • Pages from-to

    1-14

  • UT code for WoS article

    000686349300013

  • EID of the result in the Scopus database

    2-s2.0-85113376383