G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00119672" target="_blank" >RIV/00216224:14740/21:00119672 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/plphys/article/186/4/1893/6270794" target="_blank" >https://academic.oup.com/plphys/article/186/4/1893/6270794</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/plphys/kiab201" target="_blank" >10.1093/plphys/kiab201</a>
Alternative languages
Result language
angličtina
Original language name
G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability
Original language description
The WEE1 and ATM AND RAD3-RELATED (ATR) kinases are important regulators of the plant intra-S-phase checkpoint; consequently, WEE1(KO) and ATR(KO) roots are hypersensitive to replication-inhibitory drugs. Here, we report on a loss-of-function mutant allele of the FASCIATA1 (FAS1) subunit of the chromatin assembly factor 1 (CAF-1) complex that suppresses the phenotype of WEE1- or ATR-deficient Arabidopsis (Arabidopsis thaliana) plants. We demonstrate that lack of FAS1 activity results in the activation of an ATAXIA TELANGIECTASIA MUTATED (ATM)- and SUPPRESSOR OF GAMMA-RESPONSE 1 (SOG1)-mediated G2/M-arrest that renders the ATR and WEE1 checkpoint regulators redundant. This ATM activation accounts for the telomere erosion and loss of ribosomal DNA that are described for fast plants. Knocking out SOG1 in the fast weel background restores replication stress sensitivity, demonstrating that SOG1 is an important secondary checkpoint regulator in plants that fail to activate the intra-S-phase checkpoint.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Physiology
ISSN
0032-0889
e-ISSN
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Volume of the periodical
186
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
1893-1907
UT code for WoS article
000703922000018
EID of the result in the Scopus database
2-s2.0-85112243390