DNA damage-induced accumulation of centrosomal Chk1 contributes to its checkpoint function

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004909" target="_blank" >RIV/61989592:15310/07:00004909 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

DNA damage-induced accumulation of centrosomal Chk1 contributes to its checkpoint function

Original language description

The checkpoint kinase Chk1 is an established transducer of ATR- and ATM-dependent signalling in response to DNA damage. In addition to its nuclear localization, Chk1 localizes to interphase centrosomes and thereby negatively regulates entry into mitosisby preventing premature activation of cyclin B-Cdk1 during unperturbed cell cycles. Here, we demonstrate that DNA damage caused by ultraviolet irradiation or hydroxyurea treatment leads to centrosomal accumulation of endogenous Chk1 in normal human BJ fibroblasts and in ATR- or ATM-deficient fibroblasts. Chemical inhibition of ATR/ATM by caffeine led to enhanced centrosomal Chk1 deposition associated with nuclear Chk1 depletion. In contrast to normal or ATM-deficient fibroblasts, genetically ATR-deficient Seckel-fibroblasts showed detectable constitutive centrosomal accumulation of Chk1 even in the absence of exogenous insults. After DNA damage, the centrosomal fraction of Chk1 was found to be phosphorylated at ATR/ATM phosphorylation s

Czech name

Poškození DNA - indukovaná akumulace centrosomální kinasy Chk1 přispívá k její roli kontrolního bodu

Czech description

Naše výsledky ukazují, že akumulace fosphorylovaného Chk1 na centrosomech vytváří přídavný element v odpovědi na poškození DNA (DDR). Centrosomální Chk1 indukuje zastavení buněčného cyklu v G/M bodě a může evokovat centrosomální amplifikaci.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell cycle

ISSN

1538-4101

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

20

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

2541-2548

UT code for WoS article

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EID of the result in the Scopus database

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