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TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00120237" target="_blank" >RIV/00216224:14740/21:00120237 - isvavai.cz</a>

  • Alternative codes found

    RIV/68378271:_____/21:00560916

  • Result on the web

    <a href="https://www.confer.cz/nanocon/2020" target="_blank" >https://www.confer.cz/nanocon/2020</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.37904/nanocon.2020.3740" target="_blank" >10.37904/nanocon.2020.3740</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

  • Original language description

    Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM. Cell stiffness was mapped and thus monitored in time and space under nearly physiological conditions (i.e., in culture medium and at elevated temperature). In AFM force spectroscopy mode, cells are indented at many sites, and their complete elastic responses are recorded, enabling them to reconstruct a stiffness map. We measured the frozen cell surface stiffness immediately after thawing; they, when the dynamics of development of the cell stiffness were monitored in time up to 24 hours. Moreover, the AFM spectroscopy was combined with fluorescence-based staining of the cytoskeleton, thus enabling to directly correlate cytoskeleton development with stiffness mapping.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    10601 - Cell biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    CONFERENCE PROCEEDINGS - NANOCON 2020

  • ISBN

    9788087294987

  • ISSN

    2694-930X

  • e-ISSN

  • Number of pages

    6

  • Pages from-to

    416-421

  • Publisher name

    TANGER LTD

  • Place of publication

    SLEZSKA

  • Event location

    Brno

  • Event date

    Jan 1, 2020

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article

    000664505500071