Cryopreserved Cells Regeneration Monitored by Atomic Force Microscopy and Correlated With State of Cytoskeleton and Nuclear Membrane

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00101533" target="_blank" >RIV/00216224:14740/18:00101533 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/18:00497203 RIV/68378271:_____/18:00497203 RIV/00216208:11110/18:10383527 RIV/00159816:_____/18:00069355

Result on the web

<a href="http://dx.doi.org/10.1109/TNB.2018.2873425" target="_blank" >http://dx.doi.org/10.1109/TNB.2018.2873425</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1109/TNB.2018.2873425" target="_blank" >10.1109/TNB.2018.2873425</a>

Result language

angličtina

Original language name

Cryopreserved Cells Regeneration Monitored by Atomic Force Microscopy and Correlated With State of Cytoskeleton and Nuclear Membrane

Original language description

Atomic force microscopy (AFM) helps to describe and explain the mechanobiological properties of living cells on the nanoscale level under physiological conditions. The stiffness of cells is an important parameter reflecting cell physiology. Here, we have provided the first study of the stiffness of cryopreserved cells during post- thawing regeneration using AFM combined with confocal fluorescence microscopy. We demonstrated that the nonfrozen cell stiffness decreased proportionally to the cryoprotectant concentration in the medium. AFM allowed us to map cell surface reconstitution in real time after a freeze/thaw cycle and to monitor the regeneration processes at different depths of the cell and even different parts of the cell surface (nucleus and edge). Fluorescence microscopy showed that the cytoskeleton in fibroblasts, though damaged by the freeze/thaw cycle, is reconstructed after long-termplating. Confocalmicroscopy confirmed that structural changes affect the nuclear envelopes in cryopreserved cells. AFM nanoindentation analysis could be used as a noninvasive method to identify cells that have regenerated their surface mechanical properties with the proper dynamics and to a sufficient degree. This identification can be important particularly in the field of in vitro fertilization and in future cell-based regeneration strategies.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10609 - Biochemical research methods

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

IEEE TRANSACTIONS ON NANOBIOSCIENCE

ISSN

1536-1241

e-ISSN

—

Volume of the periodical

17

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

485-497

UT code for WoS article

000450357100015

EID of the result in the Scopus database

2-s2.0-85054704956