AFM Monitoring the Influence o f Selected Cryoprotectants on Regeneration of Cryopreserved Cells Mechanical Properties

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00101084" target="_blank" >RIV/00216224:14740/18:00101084 - isvavai.cz</a>
Alternative codes found
RIV/68378271:_____/18:00492480 RIV/00159816:_____/18:00069343
Result on the web
<a href="http://dx.doi.org/10.3389/fphys.2018.00804" target="_blank" >http://dx.doi.org/10.3389/fphys.2018.00804</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fphys.2018.00804" target="_blank" >10.3389/fphys.2018.00804</a>

Result language
angličtina
Original language name
AFM Monitoring the Influence o f Selected Cryoprotectants on Regeneration of Cryopreserved Cells Mechanical Properties
Original language description
Cryopreservation of cells (mouse embryonic fibroblasts) is a fundamental task for wide range of applications. In practice, cells are protected against damage during freezing by applications of specific cryoprotectants and freezing/melting protocols. In this study by using AFM and fluorescence microscopy we showed how selected cryoprotectants (dimethyl sulfoxide and polyethylene glycol) affected the cryopreserved cells mechanical properties (stiffness) and how these parameters are correlated with cytoskeleton damage and reconstruction. We showed how cryopreserved (frozen and thawed) cells' stiffness change according to type of applied cryoprotectant and its functionality in extracellular or intracellular space. We showed that AFM can be used as technique for investigation of cryopreserved cells surfaces state and development ex vivo. Our results offer a new perspective on the monitoring and characterization of frozen cells recovery by measuring changes in elastic properties by nanoindentation technique. This may lead to a new and detailed way of investigating the post-thaw development of cryopreserved cells which allows to distinguish between different cell parts.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30105 - Physiology (including cytology)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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