Human fibroblast post-thaw regeneration monitored by AFM and fluorescence microscopy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00500586" target="_blank" >RIV/68081707:_____/18:00500586 - isvavai.cz</a>
Alternative codes found
RIV/68378271:_____/18:00500586
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Human fibroblast post-thaw regeneration monitored by AFM and fluorescence microscopy
Original language description
Cryopreservation of cells (mouse embryonic fibroblasts) is a fundamental task for wide range of applications. In this study by using AFM and fluorescence microscopy we showed how selected cryoprotectants (dimethyl sulfoxide and polyethylene glycol) affected the cryopreserved cells mechanical properties (stiffness) and how these parameters are correlated with cytoskeleton damage and reconstruction. We showed how cryopreserved (frozen and thawed) cells’ stiffness change according to type of applied cryoprotectant and its functionality in extracellular or intracellular space. We showed that AFM can be used as technique for investigation of cryopreserved cells surfaces state and development ex vivo. Our results offer a new perspective on the monitoring and characterization of frozen cells recovery by measuring changes in elastic properties by nanoindentation technique
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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