Development of CE-(CD)-D-4 Method for Determination Tropane Alkaloids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00124340" target="_blank" >RIV/00216224:14740/21:00124340 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1420-3049/26/19/5749" target="_blank" >https://www.mdpi.com/1420-3049/26/19/5749</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/molecules26195749" target="_blank" >10.3390/molecules26195749</a>
Alternative languages
Result language
angličtina
Original language name
Development of CE-(CD)-D-4 Method for Determination Tropane Alkaloids
Original language description
A fast method for the determination of tropane alkaloids, using a portable CE instrument with a capacitively coupled contactless conductivity detector (CE-C4D) was developed and validated for determination of atropine and scopolamine in seeds from Solanaceae family plants. Separation was obtained within 5 min, using an optimized background electrolyte consisting of 0.5 M acetic acid with 0.25% (w/v) β-CD. The limit of detection and quantification was 0.5 µg/mL and 1.5 µg/mL, respectively, for both atropine and scopolamine. The developed method was validated with the following parameters—precision (CV): 1.07–2.08%, accuracy of the assay (recovery, RE): 101.0–102.7% and matrix effect (ME): 92.99–94.23%. Moreover, the optimized CE-C4D method was applied to the analysis of plant extracts and pharmaceuticals, proving its applicability and accuracy.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecules
ISSN
1420-3049
e-ISSN
1420-3049
Volume of the periodical
26
Issue of the periodical within the volume
19
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
5749
UT code for WoS article
000708212100001
EID of the result in the Scopus database
2-s2.0-85115665380