Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00124445" target="_blank" >RIV/00216224:14740/21:00124445 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2073-4409/10/9/2354" target="_blank" >https://www.mdpi.com/2073-4409/10/9/2354</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cells10092354" target="_blank" >10.3390/cells10092354</a>

Result language

angličtina

Original language name

Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy

Original language description

Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been documented that fully functional thylakoid membranes, in addition to the bilayer, contain an inverted hexagonal (H-II) phase and two isotropic phases. To shed light on the origin of these non-lamellar phases, we performed P-31-NMR spectroscopy experiments on sub-chloroplast particles of spinach: stacked, granum and unstacked, stroma thylakoid membranes. These membranes exhibited similar lipid polymorphism as the whole thylakoids. Saturation transfer experiments, applying saturating pulses at characteristic frequencies at 5 degrees C, provided evidence for distinct lipid phases-with component spectra very similar to those derived from mathematical deconvolution of the P-31-NMR spectra. Wheat-germ lipase treatment of samples selectively eliminated the phases exhibiting sharp isotropic peaks, suggesting easier accessibility of these lipids compared to the bilayer and the H-II phases. Gradually increasing lipid exchanges were observed between the bilayer and the two isotropic phases upon gradually elevating the temperature from 5 to 35 degrees C, suggesting close connections between these lipid phases. Data concerning the identity and structural and functional roles of different lipid phases will be presented in the accompanying paper.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

—

Continuities

—

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Blood Cells, Molecules and Diseases

ISSN

1079-9796

e-ISSN

2073-4409

Volume of the periodical

10

Issue of the periodical within the volume

9

Country of publishing house

CH - SWITZERLAND

Number of pages

16

Pages from-to

2354

UT code for WoS article

000699437700001

EID of the result in the Scopus database

2-s2.0-85115889243