Structural Entities Associated with Different Lipid Phases of Plant Thylakoid Membranes-Selective Susceptibilities to Different Lipases and Proteases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F22%3A00561387" target="_blank" >RIV/86652079:_____/22:00561387 - isvavai.cz</a>

Alternative codes found

RIV/61988987:17310/22:A2302G2Q RIV/61988987:17310/22:N2302G2Q

Result on the web

<a href="https://www.mdpi.com/2073-4409/11/17/2681" target="_blank" >https://www.mdpi.com/2073-4409/11/17/2681</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cells11172681" target="_blank" >10.3390/cells11172681</a>

Result language

angličtina

Original language name

Structural Entities Associated with Different Lipid Phases of Plant Thylakoid Membranes-Selective Susceptibilities to Different Lipases and Proteases

Original language description

It is well established that plant thylakoid membranes (TMs), in addition to a bilayer, contain two isotropic lipid phases and an inverted hexagonal (H-II) phase. To elucidate the origin of non-bilayer lipid phases, we recorded the P-31-NMR spectra of isolated spinach plastoglobuli and TMs and tested their susceptibilities to lipases and proteases, the structural and functional characteristics of TMs were monitored using biophysical techniques and CN-PAGE. Phospholipase-A1 gradually destroyed all P-31-NMR-detectable lipid phases of isolated TMs, but the weak signal of isolated plastoglobuli was not affected. Parallel with the destabilization of their lamellar phase, TMs lost their impermeability,other effects, mainly on Photosystem-II, lagged behind the destruction of the original phases. Wheat-germ lipase selectively eliminated the isotropic phases but exerted little or no effect on the structural and functional parameters of TMs-indicating that the isotropic phases are located outside the protein-rich regions and might be involved in membrane fusion. Trypsin and Proteinase K selectively suppressed the H-II phase-suggesting that a large fraction of TM lipids encapsulate stroma-side proteins or polypeptides. We conclude that-in line with the Dynamic Exchange Model-the non-bilayer lipid phases of TMs are found in subdomains separated from but interconnected with the bilayer accommodating the main components of the photosynthetic machinery.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10610 - Biophysics

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cells

ISSN

2073-4409

e-ISSN

2073-4409

Volume of the periodical

11

Issue of the periodical within the volume

17

Country of publishing house

CH - SWITZERLAND

Number of pages

18

Pages from-to

2681

UT code for WoS article

000852860900001

EID of the result in the Scopus database

2-s2.0-85137774616