Enhanced in vitro culture of leukemic cells: Insights from collagen scaffolds and carboxymethyl cellulose-polyethylene glycol gel.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00132768" target="_blank" >RIV/00216224:14740/23:00132768 - isvavai.cz</a>
Result on the web
<a href="https://www.embl.org/about/info/course-and-conference-office/events/can23-01/#vf-tabs__section-overview" target="_blank" >https://www.embl.org/about/info/course-and-conference-office/events/can23-01/#vf-tabs__section-overview</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Enhanced in vitro culture of leukemic cells: Insights from collagen scaffolds and carboxymethyl cellulose-polyethylene glycol gel.
Original language description
Studying chronic lymphocytic leukemia (CLL) in vitro is challenging due to its complexity and dependency of CLL cells on their microenvironment. An integral part of the natural CLL microenvironment is the three-dimensional (3D) spatial organization, which facilitates frequent cell-to-cell and cell-to-matrix contacts. Therefore, we aimed to mimic the natural tissue architecture by implementing a 3D in vitro culture. We hypothesized that compared to conventional culture, the additional dimension and increased cell-to-matrix contacts would enhance the prosurvival stimuli in CLL cells. We cultured CLL cells in two materials: collagen scaffolds, or gel composed of carboxymethyl cellulose and polyethylene glycol (CMC-PEG). We assessed cell distribution, morphology, and viability via microscopy, and measured the metabolic activity by AlamarBlue assay. Gene expression (MYC, VCAM1, MCL1, CXCR4, CCL4) was analyzed using qPCR to understand the effects of novel culture approaches on adhesion, apoptosis, and intercellular interactions of CLL cells co-cultured with bone marrow stromal cells (BMSCs) in 3D.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů