Hide and seek retroelement activity in hematological malignancies.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00132770" target="_blank" >RIV/00216224:14740/23:00132770 - isvavai.cz</a>
Result on the web
<a href="https://www.embl.org/about/info/course-and-conference-office/events/can23-01/" target="_blank" >https://www.embl.org/about/info/course-and-conference-office/events/can23-01/</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Hide and seek retroelement activity in hematological malignancies.
Original language description
Retroelements (REs), which function via a “copy-and-paste” mechanism, comprise nearly half of the human genome. The Long Interspersed Nucleic Elements, type 1 (LINE-1 or L1) are the only active autonomous REs. They are able to retrotranspose other RNAs including Alu and SVA REs, and occasionally protein-coding RNAs. Retroelements are silenced via multiple mechanisms, but genomic instability of cancer cells often leads to aberrant disruption of RE repression and enhances their transposition activity. The main goal of our research is to explore RE activity in chronic lymphocytic leukemia (CLL) and myelodysplastic syndrome (MDS) and to study the impact of therapy and TP53 inactivation on RE activity. To identify tumor-specific RE insertions, we adopted a highly sensitive amplicon NGS protocol for localizing insertions of REs from Alu-Ya5, Alu-Yb8, or L1-HS families into their target genomic regions. In total, 99 samples from 17 MDS and 21 CLL patients, and 60 samples from 4 leukemic cell lines were analyzed.
Czech name
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Czech description
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Project
<a href="/en/project/LX22NPO5102" target="_blank" >LX22NPO5102: National institute for cancer research</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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