Searching for transposable elements in hematological malignancies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00072426" target="_blank" >RIV/65269705:_____/19:00072426 - isvavai.cz</a>
Result on the web
<a href="http://phdretreat.ceitec.cz/joint-retreat-2019/" target="_blank" >http://phdretreat.ceitec.cz/joint-retreat-2019/</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Searching for transposable elements in hematological malignancies
Original language description
Transposable elements, or transposons, are DNA units present in eukaryotic organisms in a broad diversity of their structures. They have played an important role in evolution of many genomes. In the human genome, the vast majority of transposons is represented by retroelements (REs) that can integrate new copies through RNA-mediated mechanisms. The long interspersed nucleic elements (LINE-1 or L1) utilize a "copy-and-paste" mechanism to retrotranspose copies into new genomic loci. Active L1 retrotransposons also drive retrotransposition of other mobile DNAs, namely, Alu, short interspersed elements (SINEs), and SVA transposons. Recent findings imply that genomic instability of cancer is associated with transposon reactivation. The main goal of our research is to explore RE activity in different types of hematological malignancies. To identify tumor-specific RE insertions, we adopted a protocol based on high-throughput sequencing of amplicons containing a part of AluYa5, Alu-Ya8 or L1-HS insertions, and their adjacent genomic regions. By using unique molecular identifiers, we aim to estimate the percentage of cancer cells bearing each particular insertion. We performed a pilot experiment on 42 tumornormal sample pairs from patients with chronic lymphocytic leukemia, acute lymphoblastic leukemia, and myelodysplastic syndrome. The libraries were sequenced on Illumina NextSeq and obtained sequences are currently subjected to an in-house bioinformatics pipeline for identification of somatic RE insertions.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
30205 - Hematology
Result continuities
Project
<a href="/en/project/GA19-11299S" target="_blank" >GA19-11299S: The role of transposable element activity in hematological malignacies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů