Challenges of <i>in situ</i> hybridization in miRNA analysis of triple-negative breast cancer morphological diversity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00133481" target="_blank" >RIV/00216224:14740/23:00133481 - isvavai.cz</a>
Result on the web
<a href="http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7984&category_id=189&option=com_virtuemart&vmcchk=1&Itemid=1" target="_blank" >http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7984&category_id=189&option=com_virtuemart&vmcchk=1&Itemid=1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4149/neo_2023_220519N533" target="_blank" >10.4149/neo_2023_220519N533</a>
Alternative languages
Result language
angličtina
Original language name
Challenges of <i>in situ</i> hybridization in miRNA analysis of triple-negative breast cancer morphological diversity
Original language description
miRNA expression in triple-negative breast cancers (TNBC) has mainly been studied from a methodological viewpoint. However, it has not been considered that miRNA expression profile may be associated with a specific morphological entity inside every tumor. The verification of this hypothesis on a set of 25 TNBCs was the subject of our previous work, where we confirmed specific expression of the studied miRNAs in 82 samples of different morphologies including inflammatory infiltrate, spindle cell, clear cell, and metastases after RNA extraction and purification as well as microchip and biostatistical analysis. In the current work, we demonstrate a low suitability of in situ hybridization method for miRNA detection compared to RT-qPCR, and in detail discuss the biological role of 8 miRNAs with the most significant changes of expression.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Neoplasma
ISSN
0028-2685
e-ISSN
1338-4317
Volume of the periodical
70
Issue of the periodical within the volume
2
Country of publishing house
SK - SLOVAKIA
Number of pages
9
Pages from-to
179-187
UT code for WoS article
001070933100001
EID of the result in the Scopus database
2-s2.0-85160206806