Hairy Root Transformation and Regeneration in Arabidopsis thaliana and Brassica napus

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00134770" target="_blank" >RIV/00216224:14740/23:00134770 - isvavai.cz</a>
Result on the web
<a href="https://review.jove.com/fr/t/66223/hairy-root-transformation-regeneration-arabidopsis-thaliana-brassica?status=a68229k" target="_blank" >https://review.jove.com/fr/t/66223/hairy-root-transformation-regeneration-arabidopsis-thaliana-brassica?status=a68229k</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/66223" target="_blank" >10.3791/66223</a>

Result language
angličtina
Original language name
Hairy Root Transformation and Regeneration in Arabidopsis thaliana and Brassica napus
Original language description
Hairy root transformation represents a versatile tool for plant biotechnology in various species. Infection by an Agrobacterium strain carrying a Root-inducing (Ri) plasmid induces the formation of hairy roots at the wounding site after the transfer of T- DNA from the Ri plasmid into the plant genome. The protocol describes in detail the procedure of the injection-based hairy root induction in Brassica napus DH12075 and Arabidopsis thaliana Col-0. The hairy roots may be used to analyze a transgene of interest or processed for the generation of transgenic plants. Regeneration medium containing cytokinin 6-benzylaminopurine (5 mg/L) and auxin 1-naphthaleneacetic acid (8 mg/L) successfully elicits shoot formation in both species. The protocol covers the genotyping and selection of regenerants and T1 plants to obtain plants carrying a transgene of interest and free of T-DNA from the Ri plasmid. An alternative process leading to the formation of a composite plant is also depicted. In this case, hairy roots are kept on the shoot (instead of the natural roots), which enables the study of a transgene in hairy root cultures in the context of the whole plant.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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