Injection-based hairy root induction and plant regeneration techniques in Brassicaceae.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00135534" target="_blank" >RIV/00216224:14740/24:00135534 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1186/s13007-024-01150-1" target="_blank" >https://doi.org/10.1186/s13007-024-01150-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13007-024-01150-1" target="_blank" >10.1186/s13007-024-01150-1</a>
Alternative languages
Result language
angličtina
Original language name
Injection-based hairy root induction and plant regeneration techniques in Brassicaceae.
Original language description
Background Hairy roots constitute a valuable tissue culture system for species that are difficult to propagate through conventional seed-based methods. Moreover, the generation of transgenic plants derived from hairy roots can be facilitated by employing carefully designed hormone-containing media. Results We initiated hairy root formation in the rare crucifer species Asperuginoides axillaris via an injection-based protocol using the Agrobacterium strain C58C1 harboring a hairy root-inducing (Ri) plasmid and successfully regenerated plants from established hairy root lines. Our study confirms the genetic stability of both hairy roots and their derived regenerants and highlights their utility as a permanent source of mitotic chromosomes for cytogenetic investigations. Additionally, we have developed an effective embryo rescue protocol to circumvent seed dormancy issues in A. axillaris seeds. By using inflorescence primary stems of Arabidopsis thaliana and Cardamine hirsuta as starting material, we also established hairy root lines that were subsequently used for regeneration studies. Conclusion We developed efficient hairy root transformation and regeneration protocols for various crucifers, namely A. axillaris, A. thaliana, and C. hirsuta. Hairy roots and derived regenerants can serve as a continuous source of plant material for molecular and cytogenetic analyses.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
<a href="/en/project/LTC20004" target="_blank" >LTC20004: Insight into the transcriptional regulation of the crosstalk between plant hormones and the stress response in fruits</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLANT METHODS
ISSN
1746-4811
e-ISSN
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Volume of the periodical
20
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
112
Pages from-to
12
UT code for WoS article
001163783700001
EID of the result in the Scopus database
2-s2.0-85185244603