Primary multistep phosphorelay activation comprises both cytokinin and abiotic stress responses: insights from comparative analysis of Brassica type-A response regulators
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00137006" target="_blank" >RIV/00216224:14740/24:00137006 - isvavai.cz</a>
Result on the web
<a href="https://watermark.silverchair.com/erae335.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA2QwggNgBgkqhkiG9w0BBwagggNRMIIDTQIBADCCA0YGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMNyCYsUEbBZWAwkeqAgEQgIIDFxt-1N9lhylaG29NK2vZLUCuf3u9SwoK1h0_qEEMU4_Tug" target="_blank" >https://watermark.silverchair.com/erae335.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA2QwggNgBgkqhkiG9w0BBwagggNRMIIDTQIBADCCA0YGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMNyCYsUEbBZWAwkeqAgEQgIIDFxt-1N9lhylaG29NK2vZLUCuf3u9SwoK1h0_qEEMU4_Tug</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/erae335" target="_blank" >10.1093/jxb/erae335</a>
Alternative languages
Result language
angličtina
Original language name
Primary multistep phosphorelay activation comprises both cytokinin and abiotic stress responses: insights from comparative analysis of Brassica type-A response regulators
Original language description
Multistep phosphorelay (MSP) signaling integrates hormonal and environmental signals to control both plant development and adaptive responses. The type-A RESPONSE REGULATORs (RRAs), the downstream members of the MSP cascade and cytokinin primary response genes are supposed to mediate primarily the negative feedback regulation of (cytokinin-induced) MSP signaling. However, the transcriptional data suggest the involvement of RRAs in stress-related responses as well. By employing evolutionary conservation with the well-characterized Arabidopsis thaliana RRAs, we identified 5 and 38 novel putative RRAs in Brassica oleracea and Brassica napus, respectively. Our phylogenetic analysis suggests the existence of gene-specific selective pressure, maintaining the homologs of ARR3, ARR6, and ARR16 as singletons during the evolution of Brassicaceae. We categorized RRAs based on the kinetics of their cytokinin-mediated upregulation and observed both similarities and specificities in this type of response across Brassicaceae species. Using bioinformatic analysis and experimental data demonstrating the cytokinin and abiotic stress responsiveness of A. thaliana-derived TCSv2 reporter, we unveil the mechanistic conservation of cytokinin- and stress-mediated upregulation of RRAs in Brassica rapa and Brassica napus. Notably, we identify partial cytokinin dependency of cold stress-induced RRA transcription, thus corroborating the role of cytokinin signaling in the crop adaptive responses.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Experimental Botany
ISSN
0022-0957
e-ISSN
1460-2431
Volume of the periodical
75
Issue of the periodical within the volume
20
Country of publishing house
GB - UNITED KINGDOM
Number of pages
23
Pages from-to
6346-6368
UT code for WoS article
001302158200001
EID of the result in the Scopus database
2-s2.0-85208409578