The histone chaperones ASF1 and HIRA are required for telomere length and 45S rDNA copy number homeostasis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00137975" target="_blank" >RIV/00216224:14740/24:00137975 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26220/24:PU152202
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/tpj.17041?af=R" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/tpj.17041?af=R</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/tpj.17041" target="_blank" >10.1111/tpj.17041</a>
Alternative languages
Result language
angličtina
Original language name
The histone chaperones ASF1 and HIRA are required for telomere length and 45S rDNA copy number homeostasis
Original language description
Genome stability is significantly influenced by the precise coordination of chromatin complexes that facilitate the loading and eviction of histones from chromatin during replication, transcription, and DNA repair processes. In this study, we investigate the role of the Arabidopsis H3 histone chaperones ANTI-SILENCING FUNCTION 1 (ASF1) and HISTONE REGULATOR A (HIRA) in the maintenance of telomeres and 45S rDNA loci, genomic sites that are particularly susceptible to changes in the chromatin structure. We find that both ASF1 and HIRA are essential for telomere length regulation, as telomeres are significantly shorter in asf1a1b and hira mutants. However, these shorter telomeres remain localized around the nucleolus and exhibit a comparable relative H3 occupancy to the wild type. In addition to regulating telomere length, ASF1 and HIRA contribute to silencing 45S rRNA genes and affect their copy number. Besides, ASF1 supports global heterochromatin maintenance. Our findings also indicate that ASF1 transiently binds to the TELOMERE REPEAT BINDING 1 protein and the N terminus of telomerase in vivo, suggesting a physical link between the ASF1 histone chaperone and the telomere maintenance machinery.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Journal
ISSN
0960-7412
e-ISSN
1365-313X
Volume of the periodical
120
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
1125-1141
UT code for WoS article
001338618900001
EID of the result in the Scopus database
2-s2.0-85206236673