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Regulation of replication fork progression through histone supply and demand

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004910" target="_blank" >RIV/61989592:15310/07:00004910 - isvavai.cz</a>

  • Alternative codes found

    RIV/61989592:15110/07:00009703

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Regulation of replication fork progression through histone supply and demand

  • Original language description

    DNA replication in eukaryotes requires nucleosome disruption ahead of the replication fork and reassembly behind. An unresolved issue concerns how histone dynamics are coordinated with fork progression to maintain chromosomal stability. Here, we characterize a complex in which the human histone chaperone Asf1 and MCM2-7, the putative replicative helicase, are connected through a histone H3-H4 bridge. Depletion of Asf1 by RNA interference impedes DNA unwinding at replication sites, and similar defects arise from overproduction of new histone H3-H4 that compromises Asf1 function. These data link Asf1 chaperone function, histone supply, and replicative unwinding of DNA in chromatin. We propose that Asf1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an Asf1-(H3-H4)-MCM2-7 intermediate and thus provides a means to fine-tune replication fork progression and histone supply and demand.

  • Czech name

    Regulace progrese replikační vidličky pomocí dodání histonů

  • Czech description

    Předkládáme, že Asf1, jako akceptor a donor histonu, ovládá dceřiné a nové histony v replikační vidličce přes mezistupeň Asf1-(H3-H4)-MCM2-7 a tak poskytuje prostředky pro jemné vyladění postupu replikační vidličky a dodávku histonů.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2007

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Science

  • ISSN

    0036-8075

  • e-ISSN

  • Volume of the periodical

    318

  • Issue of the periodical within the volume

    5858

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    4

  • Pages from-to

    1928-1931

  • UT code for WoS article

  • EID of the result in the Scopus database