Regulation of replication fork progression through histone supply and demand
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004910" target="_blank" >RIV/61989592:15310/07:00004910 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/07:00009703
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Regulation of replication fork progression through histone supply and demand
Original language description
DNA replication in eukaryotes requires nucleosome disruption ahead of the replication fork and reassembly behind. An unresolved issue concerns how histone dynamics are coordinated with fork progression to maintain chromosomal stability. Here, we characterize a complex in which the human histone chaperone Asf1 and MCM2-7, the putative replicative helicase, are connected through a histone H3-H4 bridge. Depletion of Asf1 by RNA interference impedes DNA unwinding at replication sites, and similar defects arise from overproduction of new histone H3-H4 that compromises Asf1 function. These data link Asf1 chaperone function, histone supply, and replicative unwinding of DNA in chromatin. We propose that Asf1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an Asf1-(H3-H4)-MCM2-7 intermediate and thus provides a means to fine-tune replication fork progression and histone supply and demand.
Czech name
Regulace progrese replikační vidličky pomocí dodání histonů
Czech description
Předkládáme, že Asf1, jako akceptor a donor histonu, ovládá dceřiné a nové histony v replikační vidličce přes mezistupeň Asf1-(H3-H4)-MCM2-7 a tak poskytuje prostředky pro jemné vyladění postupu replikační vidličky a dodávku histonů.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Science
ISSN
0036-8075
e-ISSN
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Volume of the periodical
318
Issue of the periodical within the volume
5858
Country of publishing house
US - UNITED STATES
Number of pages
4
Pages from-to
1928-1931
UT code for WoS article
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EID of the result in the Scopus database
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