Unraveling the genetic and epigenetic impacts of large-scale chromosomal rearrangements in Arabidopsis thaliana

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00138962" target="_blank" >RIV/00216224:14740/24:00138962 - isvavai.cz</a>

Result on the web

<a href="https://meetings.embo.org/event/24-telomere" target="_blank" >https://meetings.embo.org/event/24-telomere</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Unraveling the genetic and epigenetic impacts of large-scale chromosomal rearrangements in Arabidopsis thaliana

Original language description

Chromosomal rearrangements can result from large-scale structural changes in the genome, such as inversions, translocations, duplications, and deletions. These rearrangements often have significant implications for the plant's genetic makeup and epigenetic regulation. Importantly, it is even possible to simulate large-scale genome re-arrangements mimicking those that occurred naturally during plant evolution. Using CRISPR/Cas9 approach, Arabidopsis thaliana lines with stable reciprocal translocations of chromosome arms (Beying et al., 2020) and inverted chromosome (Rönspies, 2022) were constructed. We studied phenotype, telomere lengths, levels of transcripts and chromatin structure in homozygous generations of these plants. These analyses are supposed to reveal whether and how chromatin organization is affected in response to changes in genome organization, and whether and how respective changes are inter-related. Understanding the impact of chromosomal rearrangements in Arabidopsis thaliana can provide valuable insights into the evolutionary dynamics and adaptive potential of plant genomes. Beying, N.; Schmidt, C.; Pacher, M.; Houben, A.; Puchta, H. CRISPR-Cas9-mediated induction of heritable chromosomal translocations in Arabidopsis. Nat Plants 2020, 6, 638-645. Rönspies, M.; Schmidt, C.; Schindele, P.; Lieberman-Lazarovich, M.; Houben, A.; Puchta, H. Massive crossover suppression by CRISPR-Cas-mediated plant chromosome engineering.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů