Autologous T-Cell-Free Antigen Presentation System Unveils hCMV-Specific NK Cell Response

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00139017" target="_blank" >RIV/00216224:14740/24:00139017 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2073-4409/13/6/530" target="_blank" >https://www.mdpi.com/2073-4409/13/6/530</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cells13060530" target="_blank" >10.3390/cells13060530</a>

Result language

angličtina

Original language name

Autologous T-Cell-Free Antigen Presentation System Unveils hCMV-Specific NK Cell Response

Original language description

NK cells play a decisive role in controlling hCMV infection by combining innate and adaptive-like immune reactions. The hCMV-derived VMAPRTLFL (LFL) peptide is a potent activator of NKG2C+ NK cells. Proposed here is an autologous system of LFL stimulation without T lymphocytes and exogenous cytokines that allows us to evaluate NK-cell hCMV-specific responses in more native settings. In this model, we evaluated LFL-induced IFN gamma production, focusing on signaling pathways and the degranulation and proliferation of NK cells orchestrated by microenvironment cytokine production and analyzed the transcriptome of expanded NK cells. NK cells of individuals having high anti-hCMV-IgG levels, in contrast to NK cells of hCMV-seronegative and low-positive donors, displayed increased IFN gamma production and degranulation and activation levels and enhanced proliferation upon LFL stimulation. Cytokine profiles of these LFL-stimulated cultures demonstrated a proinflammatory shift. LFL-induced NK-cell IFN gamma production was dependent on the PI3K and Ras/Raf/Mek signaling pathways, independently of cytokines. In hCMV-seropositive individuals, this model allowed obtaining NK-cell antigen-specific populations proliferating in response to LFL. The transcriptomic profile of these expanded NK cells showed increased adaptive gene expression and metabolic activation. The results complement the existing knowledge about hCMV-specific NK-cell response. This model may be further exploited for the identification and characterization of antigen-specific NK cells.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Advances in Fuel Cells

ISSN

2073-4409

e-ISSN

—

Volume of the periodical

13

Issue of the periodical within the volume

6

Country of publishing house

CH - SWITZERLAND

Number of pages

26

Pages from-to

1-26

UT code for WoS article

001191888500001

EID of the result in the Scopus database

2-s2.0-85188681939