MONOPTEROS isoform MP11ir plays a role during somatic embryogenesis in Arabidopsis thaliana
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00142788" target="_blank" >RIV/00216224:14740/25:00142788 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1093/plphys/kiaf602" target="_blank" >https://doi.org/10.1093/plphys/kiaf602</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/plphys/kiaf602" target="_blank" >10.1093/plphys/kiaf602</a>
Alternative languages
Result language
angličtina
Original language name
MONOPTEROS isoform MP11ir plays a role during somatic embryogenesis in Arabidopsis thaliana
Original language description
Auxin is crucial for plant morphogenesis, including embryo development. Exogenous auxin application is necessary for inducing embryogenic responses in in vitro cultured explants of Arabidopsis (Arabidopsis thaliana) and other plants. Thus, components of auxin transport, signaling, and metabolism are key to somatic embryogenesis. AUXIN RESPONSE FACTOR (ARF) transcription factors bind to auxin response elements to control auxin-responsive gene expression and are often repressed by AUXIN/INDOLE-3-ACETIC ACIDs (Aux/IAAs). MONOPTEROS (MP)/ARF5 is especially important in the embryogenic transition, being highly expressed during somatic embryogenesis; its mutant cannot develop somatic embryos. The MP11ir transcript, an alternatively spliced variant of MP, produces a truncated protein missing the Phox and Bem1p (PB1) domain, crucial for ARF-Aux/IAA dimerization. This renders MP11ir insensitive to Aux/IAA repression, suggesting auxin-independent regulation. High levels of MP11ir transcript are observed during auxin- and trichostatin-A-dependent induction of somatic embryogenesis. Both MP and MP11ir are essential for embryo regeneration in the mpS319 mutant. However, overexpression of a truncated MP protein (Delta ARF5) lacking the PB1 domain inhibits somatic embryogenesis, resulting in callus instead of somatic embryos. Overexpression of Delta ARF5, lack of MP protein (mp mutant), or interference with MP action by the auxin-resistant BODENLOS (BDL) protein affects the expression of auxin biosynthesis genes. Our results suggest that these auxin-related genes might be targets of MP11ir and/or MP. Consequently, any adjustment to MP activity alters auxin homeostasis and endogenous auxin levels, hindering embryogenic transition.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLANT PHYSIOLOGY
ISSN
0032-0889
e-ISSN
1532-2548
Volume of the periodical
199
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
1-20
UT code for WoS article
001628596400001
EID of the result in the Scopus database
2-s2.0-105023547420