Adenosine (de)methylation on the crossroads of RNA and DNA synthesis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00143654" target="_blank" >RIV/00216224:14740/25:00143654 - isvavai.cz</a>
Result on the web
<a href="https://www.oeaw.ac.at/imba/seminars-events/microsymposium" target="_blank" >https://www.oeaw.ac.at/imba/seminars-events/microsymposium</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Adenosine (de)methylation on the crossroads of RNA and DNA synthesis
Original language description
Eukaryotic mRNAs are subject to significant chemical modifications that exhibit a multi-layered regulation of transcript metabolism. Among these, N6-methyladenosine (m6A) and N6-2’O-dimethyladenosine (m6Am) are two prevalent modifications in higher eukaryotes. The dynamics of these modifications are tightly governed by dedicated machineries, the perturbation of which destabilise cellular homeostasis. In a recent study using proximity-based labelling approach, we identified that m6A/m eraser protein – FTO – was proximally interacting with proteins in DNA replication and repair pathways. To elucidate this further, in this study we performed subsequent protein interaction assays and genome wide CRISPR screening, where we identified multiple candidates involved at different levels of DNA synthesis. Additionally, we performed functional assays to further investigate the relevance of FTO in these processes. Preliminary results from these functional assays positively correlate with our observations from the high throughput screening approaches, highlighting the importance of the m6A/m modifying enzyme, FTO, in the maintenance of genome integrity.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EH22_008%2F0004575" target="_blank" >EH22_008/0004575: RNA for therapy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů