Unraveling Protein-Protein Interactions with Precision: Insights from FLIM-FRET Technique
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00143777" target="_blank" >RIV/00216224:14740/25:00143777 - isvavai.cz</a>
Result on the web
<a href="https://olomouc.ueb.cas.cz/en/cpnw-programme" target="_blank" >https://olomouc.ueb.cas.cz/en/cpnw-programme</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Unraveling Protein-Protein Interactions with Precision: Insights from FLIM-FRET Technique
Original language description
FLIM-FRET (Fluorescence Lifetime Imaging Microscopy–Förster Resonance Energy Transfer) analysis has revolutionized the study of plant protein complexes by allowing real-time, high-resolution visualization and quantification of protein-protein interactions in living cells. By precisely measuring fluorescence lifetime changes, this technique provides a robust platform for dissecting the molecular architecture of nuclear protein assemblies and dynamic signaling pathways. In this study, we first demonstrate how Telomere Repeat-Binding proteins in Physcomitrella patens assemble into distinct nuclear complexes via homo- and heterodimerization in nucleoplasmic foci. We then apply FLIM-FRET to investigate cytokinin signaling events by showing that phosphorylation of Arabidopsis Histidine Phosphotransfer Protein 3 at a specific serine motif critically influences its binding to other cytokinin signaling components. Collectively, these findings underscore the versatility of FLIM-FRET for studying both dynamic protein complexes and signaling pathways, offering new insights into the molecular mechanisms under various physiological and environmental conditions.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/LUAUS24277" target="_blank" >LUAUS24277: Elucidating mechanisms of hormonal control over the longitudinal root zonation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů