Advanced fluorescence imaging course on FLIM and FCS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00113569" target="_blank" >RIV/00216224:14740/19:00113569 - isvavai.cz</a>

Result on the web

<a href="https://www.ceitec.eu/advanced-fluorescence-imaging-course-on-flim-and-fcs/a3705" target="_blank" >https://www.ceitec.eu/advanced-fluorescence-imaging-course-on-flim-and-fcs/a3705</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Advanced fluorescence imaging course on FLIM and FCS

Original language description

There are a lot of in vitro techniques to study small molecular interactions, but most of them fail to study the same under in vivo conditions. Advanced fluorescence imaging is an excellent for this purpose. Apart from the initially used property of photon intensity in fluorescence imaging studies, advanced imaging techniques use fluorescence properties such as fluorescence lifetime, correlation of movement of fluorescent molecules, total internal reflection or structured illumination approaches. Here we introduce the advanced fluorescent imaging techniques FLIM-FRET and FCS. FLIM-FRET is combination of fluorescence lifetime imaging microscopy (FLIM) and Förster resonance energy transfer (FRET). Fluorescent correlation and cross-correlation spectroscopy (FCS, FCCS) are techniques that allow us to study the fluorescent molecule concentration and diffusion at the single molecule level. FCCS is excellent for description of complex formation between fast moving molecules transferring across the confocal volume of a microscope.

Czech name

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Czech description

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Type

W - Workshop organization

CEP classification

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OECD FORD branch

10609 - Biochemical research methods

Project

<a href="/en/project/LM2015062" target="_blank" >LM2015062: National Infrastructure for Biological and Medical Imaging</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Event location

Brno

Event country

CZ - CZECH REPUBLIC

Event starting date

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Event ending date

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Total number of attendees

28

Foreign attendee count

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Type of event by attendee nationality

CST - Celostátní akce