Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00143823" target="_blank" >RIV/00216224:14740/25:00143823 - isvavai.cz</a>

Result on the web

<a href="https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1612366/full" target="_blank" >https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1612366/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2025.1612366" target="_blank" >10.3389/fpls.2025.1612366</a>

Result language

angličtina

Original language name

Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties

Original language description

Introduction Acid-growth theory has been postulated in the 70s to explain the rapid elongation of plant cells in response to the hormone auxin. More recently, it has been demonstrated that activation of the proton ATPs pump (H+-ATPs) promoting acidification of the apoplast is the principal mechanism by which auxin and other hormones such as brassinosteroids (BR) induce cell elongation. Despite these advances, the impact of this acidification on the mechanical properties of the cell wall remained largely unexplored.Methods Here, we use elongation assays of Arabidopsis thaliana hypocotyls and Atomic Force Microscopy (AFM) to correlate hormone-induced tissue elongation and local changes in cell wall mechanical properties. Furthermore, employing transgenic lines over-expressing Pectin Methyl Esterase (PME), along with calcium chelators, we investigate the effect of pectin modification in hormone-driven cell elongation.Results We demonstrate that acidification of apoplast is necessary and sufficient to induce cell elongation through promoting cell wall softening. Moreover, we show that enhanced PME activity can induce both cell wall softening or stiffening in extracellular calcium dependent-manner and that tight control of PME activity is required for proper hypocotyl elongation.Discussion Our results confirm a dual role of PME in plant cell elongation. However, further investigation is needed to assess the status of pectin following short- or long-term PME treatments in order to determine if pectin methyl-esterification might promote its degradation as well as the role of PME inhibitors upon PME induction.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

—

Continuities

—

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

—

Volume of the periodical

16

Issue of the periodical within the volume

Jul

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

1-14

UT code for WoS article

001530690900001

EID of the result in the Scopus database

2-s2.0-105010905399