Click chemistry-based tracking reveals putative cell wall-located auxin binding sites in expanding cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00485861" target="_blank" >RIV/61389030:_____/17:00485861 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/17:00095528
Result on the web
<a href="http://dx.doi.org/10.1038/s41598-017-16281-w" target="_blank" >http://dx.doi.org/10.1038/s41598-017-16281-w</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-017-16281-w" target="_blank" >10.1038/s41598-017-16281-w</a>

Result language
angličtina
Original language name
Click chemistry-based tracking reveals putative cell wall-located auxin binding sites in expanding cells
Original language description
Auxin is a key plant regulatory molecule, which acts upon a plethora of cellular processes, including those related to cell differentiation and elongation. Despite the stunning progress in all disciplines of auxin research, the mechanisms of auxin-mediated rapid promotion of cell expansion and underlying rearrangement of cell wall components are poorly understood. This is partly due to the limitations of current methodologies for probing auxin. Here we describe a click chemistry-based approach, using an azido derivative of indole-3-propionic acid. This compound is as an active auxin analogue, which can be tagged in situ. Using this new tool, we demonstrate the existence of putative auxin binding sites in the cell walls of expanding/elongating cells. These binding sites are of protein nature but are distinct from those provided by the extensively studied AUXIN BINDING PROTEIN 1 (ABP1). Using immunohistochemistry, we have shown the apoplastic presence of endogenous auxin epitopes recognised by an anti-IAA antibody. Our results are intriguingly in line with previous observations suggesting some transcription-independent (non-genomic) activity of auxin in cell elongation.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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