PWO proteins are associated with PRC2 since their emergence in vascular plants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F26%3A00144778" target="_blank" >RIV/00216224:14740/26:00144778 - isvavai.cz</a>
Result on the web
<a href="https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.71172" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.71172</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.71172" target="_blank" >10.1111/nph.71172</a>
Alternative languages
Result language
angličtina
Original language name
PWO proteins are associated with PRC2 since their emergence in vascular plants
Original language description
The Polycomb repressive complex 2 (PRC2), a conserved histone methyltransferase complex, plays a central role in transcriptional silencing across eukaryotes. Here, we investigate the evolution of PWWP-DOMAIN INTERACTORS OF POLYCOMBS (PWOs), which interact with PRC2, and examine the conservation of the PWOs-PRC2 interaction across plant evolution by comparing orthologs from the lycophyte Selaginella moellendorffii (Sm) and the flowering plant Arabidopsis thaliana (Arabidopsis; At). Phylogenetic data traced the presence of PWO proteins across plant lineages, while protein-protein interaction assays and AlphaFold predictions assessed PWO-PRC2 interactions and structural conservation. Functional complementation assays confirmed PWOs' conserved functions. PWO proteins are present in vascular plants but absent in bryophytes and green algae. The ancestral clade of PWO proteins includes the spikemoss Selaginella moellendorffii (Sm) PWO orthologs SmPWOa and SmPWOb. PWO proteins from vascular plants can interact with PRC2 components from multiple species, including bryophytes, which naturally lack PWOs. The PWO-PRC2 interaction is mediated by a conserved short C-motif. Functional and molecular assays further demonstrate that SmPWOs retain their conserved functions in Arabidopsis. Our data suggest an evolutionarily conserved role for PWOs in modulating PRC2 activity and emphasize how lineage-specific proteins associated with conserved chromatin-modifying complexes may shape epigenetic control mechanisms during evolution.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2026
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
NEW PHYTOLOGIST
ISSN
0028-646X
e-ISSN
1469-8137
Volume of the periodical
250
Issue of the periodical within the volume
6
Country of publishing house
GB - UNITED KINGDOM
Number of pages
20
Pages from-to
4002-4021
UT code for WoS article
001744307700001
EID of the result in the Scopus database
2-s2.0-105036291511