Epitope extraction technique using a proteolytic microreactor combined with FTICR-MS as a tool for high-throughput screening of potential vaccine lead proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F05%3A00002641" target="_blank" >RIV/00216275:25310/05:00002641 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Epitope extraction technique using a proteolytic microreactor combined with FTICR-MS as a tool for high-throughput screening of potential vaccine lead proteins

Original language description

Epitope extraction technique is based on the specific digestion of a target protein followed by immunoaffinity isolation of a specific recognition peptide. This technique, in combination with mass spectrometry, has been efficiently used for epitope identification. The major goal of this work was to utilize newly developed enzyme and immunoaffinity magnetic reactors for the epitope extraction procedure and confirm the efficiency of this improved system for epitope screening of proteins. Alginic acid-coated magnetite microparticles with immobilized TPCK-trypsin provided high working efficiency with low non-specific adsorption, digestion time in minutes and low frequency of missed cleavages. The sensitivity and specificity of tryptic fragmentation of thebeta-amyloid-peptide Abeta (1-40) as a model polypeptide was confirmed by Fourier-transform ion cyclotron resonance mass spectrometry analysis. The Sepharose reactor or immunoaffinity magnetic reactors, both with anti-amyloid-beta monoclo

Czech name

Extrakce epitopů - nástroj ke screeningu potencionálních peptidů vhodných pro vakcínu, za použití proteolytického magnetického reaktoru kombinovaného s FT-ICR MS.

Czech description

Extrakce epitopů - nástroj ke screeningu potencionálních peptidů vhodných pro vakcínu, za použití proteolytického magnetického reaktoru kombinovaného s FT-ICR MS.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F05%2F0241" target="_blank" >GA203/05/0241: Development of bioaffinity reactors for on-chip determination and analysis of epitopes with immunogenic or allergogenic potencial</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Mass Spectrometry

ISSN

1469-0667

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

489-495

UT code for WoS article

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EID of the result in the Scopus database

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