Benefits of immunomagnetic separation for epitope identification in clinically important protein antigens: a case study using ovalbumin, carbonic anhydrase I and tau Protein

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900558" target="_blank" >RIV/00216275:25310/15:39900558 - isvavai.cz</a>

Alternative codes found

RIV/00023752:_____/15:43914723

Result on the web

<a href="http://www.hrpub.org/download/20150301/UJBE1-10603408.pdf" target="_blank" >http://www.hrpub.org/download/20150301/UJBE1-10603408.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Benefits of immunomagnetic separation for epitope identification in clinically important protein antigens: a case study using ovalbumin, carbonic anhydrase I and tau Protein

Original language description

Immunomagnetic separation (IMS) with specific antibody as affinity ligand immobilized on a magnetic carrier has several advantages in comparison with standard column separation procedures. Epitope mapping enabling identification and characterization of protein structures reactive with the antibody represents one possible application of IMS. We used epitope extraction technique based on the proteolytic digestion of the target protein followed by capturing of a specific peptide fragments by the antibody immobilized on the solid phase. Magnetic particles coated with antibody molecules were first incubated with the prepared mixture of peptides. after specific binding of peptide fragments comprising the epitope sequences, the beads were washed to remove non-epitope peptides. Captured epitope-peptides were then eluted in small volume of 0.05% TFA. Elution fractions were finally analyzed without any modification by mass spectrometry. In this work the results and experience gained in epitope m

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Universal Journal of Biomedical Engineering

ISSN

2333-2662

e-ISSN

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Volume of the periodical

3

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

1-8

UT code for WoS article

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EID of the result in the Scopus database

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