Is it correct to leave out the fluorimetric methods for glutathione assay?

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F05%3A00003016" target="_blank" >RIV/00216275:25310/05:00003016 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Is it correct to leave out the fluorimetric methods for glutathione assay?
Original language description
Glutathione is the main intracellular antioxidant, which exists in two forms, the reduced and the oxidized. Changes in glutathione concentration serve as an important marker of the redox state inside the cells. Various methods can be used to measure glutathione concentration in biological material, namely enzymatic, fluorimetric or HPLC. Nevertheless, due to the high sensitivity and specificity HPLC methods are preferred to analyze glutathione concentration, in spite of high price and time demand. The aim of our study was to establish analytical parameters of the spectrofluorimetric method, which should be suitable for the analysis of huge amount of samples. This method is based on the well-known principle - reaction between glutathione and o phthaldialdehyde with subsequent production of strongly fluorescent isoindole-like product. To confirm selectivity of this method, we have analysed glutathione concentration in hepatocyte lysates using HPLC method with spectrofluorimetric detectio
Czech name
Je správné opomíjet fluorimetrické metody při stanovení glutathionu?
Czech description
V práci byly stanoveny analytické parametry spektrofluorimetrického stanovení glutathionu, které prokázaly vhodnost metody pro stanovení glutathionu v biologickém materiálu.

Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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