Occurrence of Arcobacters in Czech Republic and the influence of sample matrix on their detection using PCR.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F06%3A00004122" target="_blank" >RIV/00216275:25310/06:00004122 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Occurrence of Arcobacters in Czech Republic and the influence of sample matrix on their detection using PCR.
Original language description
Sixty nine meat and water samples were examined by m-PCR assay of the enrichment media. Twenty nine (42 %) of samples were contaminated with Arcobacter species. In 17 samples (24,6 %), A. butzleri was confirmed by m-PCR. Direct detection of arcobacters from assay avoided cultivation on agar plates and reduced the time required for detection. Sensitivity of optimised m-PCR method was verified using four different enrichment broths artificially contaminated with pure cultures of Arcobacters. Formula 6 gave the best results from recommended enrichment media. Influence of matrix samples was tested on real samples of chicken, pork and beef, in which suspension of definite density of Arcobacters was added. It was shown, particularly, that a sample with higher fat content has required a longer incubation period in enrichment broth for successful detection by m-PCR assay.
Czech name
Výskyt arkobakterů v ČR a vliv matrice vzorku na jejich detekci metodou PCR.
Czech description
Výskyt arkobakterů v ČR a vliv matrice vzorku na jejich detekci metodou PCR.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Modern instrumentation in chemical, food and clinical analysis</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Food and Nutrition Research
ISSN
1336-8672
e-ISSN
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Volume of the periodical
45
Issue of the periodical within the volume
4
Country of publishing house
SK - SLOVAKIA
Number of pages
7
Pages from-to
152-158
UT code for WoS article
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EID of the result in the Scopus database
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